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A real-time PCR assay for the in vitro qualitative direct detection of the toxin B gene (tcdB) of C. difficile. Extraction and amplification is performed in one protocol with no separate nucleic acid extraction step.

3M Integrated Cycler 110V, laptop computer, accessories box, and optional printer

For amplifying the 5' region of the gene for adenovirus hexon polypeptide with a FAM labeled forward primer.

For amplifying the IS1001 insertion sequence with a CAL Flour Red 610 labeled forward primer.

For amplifying the IS481 insertion sequence with a FAM labeled forward primer.

For amplifying the VP2 gene with a FAM labeled forward primer.

For amplifying a well conserved region of the flagellin gene with a FAM labeled forward primer.

For amplification and detection of toxin B gene with a FAM labeled forward primer.

For amplifying a well conserved region of the Cpn0980 gene with a FAM labeled forward primer.

For amplifying CMV UL83 gene with a FAM labeled forward primer.